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The thymidine analog 5-bromo-2′-deoxyuridine (BrdU) is used to label dividing cells. BrdU is injected intraperitoneally and incorporates into DNA being replicated where it will remain for the life of the cell.
Radioactive labelling techniques are of major importance in the study of DNA synthesis in bacteria. In order to label DNA in Escherichia coli either radioactive thymine or thymidine is generally used because these compounds are specifically incorporated into DNA.
Thymidine is a pyrimidine 2'-deoxyribonucleoside having thymine as the nucleobase. ... Thymidine is a pyrimidine deoxynucleoside. Thymidine is the DNA nucleoside T, which pairs with deoxyadenosine (A) in double-stranded DNA.
High concentrations of thymidine interrupt the deoxynucleotide metabolism pathway through competitive inhibition, thus blocking DNA replication. A single treatment with thymidine arrests cells throughout S phase, so a double treatment acts to induce a more uniform block in early S phase.