Title
TRICINE N TRIS(HYDROXYMETHYL METHYL GLYCINE
SKU
RXSOL-60-6605-763
Supply Location::
Locations
TRICINE N TRIS(HYDROXYMETHYL METHYL GLYCINE manufacturers, suppliers, exporters in Mumbai, Gandhidham, Kolkata, Varanasi, Visakhapatnam, Chennai, Fujairah, Dubai, Canada BC, Barka, Sohar, Muscat, Oman.
Lab chemicals manufacturers, suppliers, exporters in India, UAE Middle East, Barka, Sohar, Muscat, Oman, Canada.
TRICINE N TRIS(HYDROXYMETHYL METHYL GLYCINE is available in small packing as well as in bulk. Buy premium quality TRICINE N TRIS(HYDROXYMETHYL METHYL GLYCINE and other lab chemicals from one of the most trusted brands.
Category
Lab Chemical-60
SHORT DESCRIPTION:::
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FIELD NOTE:::
Tricine is a commonly used electrophoresis buffer and is also used for the resuspension of cell pellets. It has a lower negative charge than glycine, allowing it to migrate faster. In addition, its high ionic strength causes more ion movement and less protein movement.
Note
Tricine is an organic compound that is used in buffer solutions. The name tricine comes from tris and glycine, from which it was derived. It is a white crystalline powder that is moderately soluble in water. It is a zwitterionic amino acid that has a pKa1 value of 2.3 at 25 °C, while its pKa2 at 20 °C is 8.15.
Application
Tricine gels are suitable in isolating hydrophobic proteins from 2D gel for mass spectrophotometric analysis. It is also helpful to isolate membrane protein complexes from biological membranes. When urea is added in stacking gel it can easily separate two different proteins of the same molecular weight
Dose
Glycine is used as a bulking agent buffers. Glycine at low concentrations prevents pH decrease in solutions. It also stabilizes a protein when present in an amorphous
