Title
FIELD STAIN B FOR MICROSCOPIAL STAINING
SKU
RXSOL-60-6604-598
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Category
Lab Chemical-60
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FIELD NOTE:::
FIELD STAIN B FOR MICROSCOPIAL STAINING This SOP should be used in conjunction with the series of other SOPs Cover the slide with 1 mL of diluted Field's stain B .
Note
The neutral component of the cells are stained by both the dyes. This staining method is used for screening thick films of malarial parasites.
Application
Field Stains contain methylene blue and eosin. These basic and acidic dyes induce multiple colours when applied to cells. The fixative, methanol does not allow any further change in slide. The basic component of white cells (cytoplasm) is stained by
acidic dye and they are described as eosinophilic or acidophilic.Field Stains contain methylene blue and eosin. These basic and acidic dyes induce multiple colours when applied to cells. The
fixative, methanol does not allow any further change in slide. The basic component of white cells (cytoplasm) is stained by
acidic dye and they are described as eosinophilic or acidophilic.Field Stains contain methylene blue and eosin. These basic and acidic dyes induce multiple colours when applied to cells. The
fixative, methanol does not allow any further change in slide. The basic component of white cells (cytoplasm) is stained by
acidic dye and they are described as eosinophilic or acidophilic.
Dose
Dry the blood film and immerse in methanol for 2-3 minutes.Blow off with Fields Stain A (S008) for 2-3 seconds. Wash it with distilled water, and again blow with Fields Stain B (S009) for 2-3 seconds and wash with distilled water. Dry it and observe under microscope.
